Upstream start codon in segment 4 of North American H2 avian influenza A viruses.
Identifieur interne : 000063 ( 1957/Analysis ); précédent : 000062; suivant : 000064Upstream start codon in segment 4 of North American H2 avian influenza A viruses.
Auteurs : Geneviève Girard [Pays-Bas] ; Alexander P. Gultyaev ; René C L. OlsthoornSource :
- Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [ 1567-7257 ] ; 2011.
Descripteurs français
- KwdFr :
- ARN messager (génétique), Alignement de séquences, Amérique du Nord, Animaux, Cellules HeLa, Codon d'initiation, Glycoprotéine hémagglutinine du virus influenza (génétique), Grippe chez les oiseaux (virologie), Grippe humaine (virologie), Gènes rapporteurs, Gènes viraux, Humains, Oiseaux, Pandémies, Phylogénie, Sous-type H2N2 du virus de la grippe A (génétique).
- MESH :
- génétique : ARN messager, Glycoprotéine hémagglutinine du virus influenza, Sous-type H2N2 du virus de la grippe A.
- virologie : Grippe chez les oiseaux, Grippe humaine.
- Alignement de séquences, Amérique du Nord, Animaux, Cellules HeLa, Codon d'initiation, Gènes rapporteurs, Gènes viraux, Humains, Oiseaux, Pandémies, Phylogénie.
English descriptors
- KwdEn :
- Animals, Birds, Codon, Initiator, Genes, Reporter, Genes, Viral, HeLa Cells, Hemagglutinin Glycoproteins, Influenza Virus (genetics), Humans, Influenza A Virus, H2N2 Subtype (genetics), Influenza in Birds (virology), Influenza, Human (virology), North America, Pandemics, Phylogeny, RNA, Messenger (genetics), Sequence Alignment.
- MESH :
- chemical , genetics : Hemagglutinin Glycoproteins, Influenza Virus, RNA, Messenger.
- chemical : Codon, Initiator.
- geographic : North America.
- genetics : Influenza A Virus, H2N2 Subtype.
- virology : Influenza in Birds, Influenza, Human.
- Animals, Birds, Genes, Reporter, Genes, Viral, HeLa Cells, Humans, Pandemics, Phylogeny, Sequence Alignment.
Abstract
H2N2 influenza A virus was the cause of the 1957 pandemic. Due to its constant presence in birds, the H2 subtype remains a topic of interest. In this work, comparison of H2 leader sequences of influenza A segment 4 revealed the presence of an upstream in-frame start codon in a majority of North American avian strains. This AUG is located seven codons upstream of the conventional start codon and is in a good Kozak context. In vivo experiments, using a luciferase reporter gene fused to leader sequences derived from North American avian H2 strains, support the efficient use of the upstream start codon. These results were corroborated by in vitro translation data using full-length segment 4 mRNA. Phylogenic analyses indicate that the upstream AUG, first detected in 1976, is stably nested in the North American avian lineage of H2 strains nowadays. The possible consequences of the upstream AUG are discussed.
DOI: 10.1016/j.meegid.2010.12.014
PubMed: 21232632
Affiliations:
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pubmed:21232632Le document en format XML
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Gultyaev</name></author><author><name sortKey="Olsthoorn, Rene C L" sort="Olsthoorn, Rene C L" uniqKey="Olsthoorn R" first="René C L" last="Olsthoorn">René C L. 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Box 9502, 2300 RA Leiden, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden</wicri:regionArea><wicri:noRegion>2300 RA Leiden</wicri:noRegion></affiliation></author><author><name sortKey="Gultyaev, Alexander P" sort="Gultyaev, Alexander P" uniqKey="Gultyaev A" first="Alexander P" last="Gultyaev">Alexander P. Gultyaev</name></author><author><name sortKey="Olsthoorn, Rene C L" sort="Olsthoorn, Rene C L" uniqKey="Olsthoorn R" first="René C L" last="Olsthoorn">René C L. Olsthoorn</name></author></analytic><series><title level="j">Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases</title><idno type="eISSN">1567-7257</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Birds</term><term>Codon, Initiator</term><term>Genes, Reporter</term><term>Genes, Viral</term><term>HeLa Cells</term><term>Hemagglutinin Glycoproteins, Influenza Virus (genetics)</term><term>Humans</term><term>Influenza A Virus, H2N2 Subtype (genetics)</term><term>Influenza in Birds (virology)</term><term>Influenza, Human (virology)</term><term>North America</term><term>Pandemics</term><term>Phylogeny</term><term>RNA, Messenger (genetics)</term><term>Sequence Alignment</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (génétique)</term><term>Alignement de séquences</term><term>Amérique du Nord</term><term>Animaux</term><term>Cellules HeLa</term><term>Codon d'initiation</term><term>Glycoprotéine hémagglutinine du virus influenza (génétique)</term><term>Grippe chez les oiseaux (virologie)</term><term>Grippe humaine (virologie)</term><term>Gènes rapporteurs</term><term>Gènes viraux</term><term>Humains</term><term>Oiseaux</term><term>Pandémies</term><term>Phylogénie</term><term>Sous-type H2N2 du virus de la grippe A (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Hemagglutinin Glycoproteins, Influenza Virus</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Codon, Initiator</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>North America</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A Virus, H2N2 Subtype</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN messager</term><term>Glycoprotéine hémagglutinine du virus influenza</term><term>Sous-type H2N2 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe chez les oiseaux</term><term>Grippe humaine</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Influenza in Birds</term><term>Influenza, Human</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Birds</term><term>Genes, Reporter</term><term>Genes, Viral</term><term>HeLa Cells</term><term>Humans</term><term>Pandemics</term><term>Phylogeny</term><term>Sequence Alignment</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Amérique du Nord</term><term>Animaux</term><term>Cellules HeLa</term><term>Codon d'initiation</term><term>Gènes rapporteurs</term><term>Gènes viraux</term><term>Humains</term><term>Oiseaux</term><term>Pandémies</term><term>Phylogénie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">H2N2 influenza A virus was the cause of the 1957 pandemic. Due to its constant presence in birds, the H2 subtype remains a topic of interest. In this work, comparison of H2 leader sequences of influenza A segment 4 revealed the presence of an upstream in-frame start codon in a majority of North American avian strains. This AUG is located seven codons upstream of the conventional start codon and is in a good Kozak context. In vivo experiments, using a luciferase reporter gene fused to leader sequences derived from North American avian H2 strains, support the efficient use of the upstream start codon. These results were corroborated by in vitro translation data using full-length segment 4 mRNA. Phylogenic analyses indicate that the upstream AUG, first detected in 1976, is stably nested in the North American avian lineage of H2 strains nowadays. The possible consequences of the upstream AUG are discussed.</div></front></TEI><affiliations><list><country><li>Pays-Bas</li></country></list><tree><noCountry><name sortKey="Gultyaev, Alexander P" sort="Gultyaev, Alexander P" uniqKey="Gultyaev A" first="Alexander P" last="Gultyaev">Alexander P. Gultyaev</name><name sortKey="Olsthoorn, Rene C L" sort="Olsthoorn, Rene C L" uniqKey="Olsthoorn R" first="René C L" last="Olsthoorn">René C L. Olsthoorn</name></noCountry><country name="Pays-Bas"><noRegion><name sortKey="Girard, Genevieve" sort="Girard, Genevieve" uniqKey="Girard G" first="Geneviève" last="Girard">Geneviève Girard</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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